An electrochemical immunosensor for the corona virus associated with the Middle East respiratory syndrome using an array of gold nanoparticle-modified carbon electrodes

Laila Ali Layqah, Shimaa Eissa

Research output: Contribution to journalArticlepeer-review

300 Scopus citations

Abstract

The Middle East respiratory syndrome corona virus (MERS-CoV) is highly pathogenic. An immunosensor for the determination of MERS-CoV is described here. It is based on a competitive assay carried out on an array of carbon electrodes (DEP) modified with gold nanoparticles. Recombinant spike protein S1 was used as a biomarker for MERS CoV. The electrode array enables multiplexed detection of different CoVs. The biosensor is based on indirect competition between free virus in the sample and immobilized MERS-CoV protein for a fixed concentration of antibody added to the sample. Voltammetric response is detected by monitoring the change in the peak current (typically acquired at a working potential of −0.05 V vs. Ag/AgCl) after addition of different concentrations of antigen against MERS-CoV. Electrochemical measurements using ferrocyanide/ferricyanide as a probe were recorded using square wave voltammetry (SWV). Good linear response between the sensor response and the concentrations from 0.001 to 100 ng.mL −1 and 0.01 to 10,000 ng.mL −1 were observed for MERS-CoV and HCoV, respectively. The assay was performed in 20 min with detection limit as low as 0.4 and 1.0 pg.mL −1 for HCoV and MERS-CoV, respectively. The method is highly selective over non-specific proteins such as Influenza A and B. The method is single-step, sensitive and accurate. It was successfully applied to spiked nasal samples.

Original languageBritish English
Article number224
JournalMicrochimica Acta
Volume186
Issue number4
DOIs
StatePublished - 1 Apr 2019

Keywords

  • Array electrode
  • Competitive immunosensor
  • Corona virus
  • Electrochemical biosensor
  • HCoV
  • MERS-CoV
  • Multiplexed biosensor
  • Simultaneous detection
  • Voltammetry

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