An efficient and rapid method to extract and purify protein – Liquid Triphasic Flotation system

Shir Reen Chia, Kah Yan Mak, Yee Jian Khaw, Nazeem Suhaidi, Kit Wayne Chew, Pau Loke Show

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Microalgae are rich in valuable biomolecules and grow on non-arable land with rapid growth rate, which has a host of new possibility as alternative protein sources. In the present study, extraction of proteins from Chlorella vulgaris via an efficient technique, Liquid Triphasic Flotation (LTF) system, was studied. The optimized conditions in LTF system were 70% v/v of t-butanol, 40% w/v of salt solution, 0.5% w/v of biomass, pH 5.54, 1:1 of salt to t-butanol solution, and 10 min of air flotation time to attain 87.23% of protein recovery and 56.72% of separation efficiency. Besides, the study on recycling t-butanol has demonstrated that only one run was sufficient to maintain the performance of system. The efficiency of LTF in extracting protein has performed better than just Three Phase Partitioning (TPP) system. LTF system is hence an effective protein extraction and purification method with minimum operation unit and processing time.

Original languageBritish English
Article number122158
JournalBioresource Technology
Volume294
DOIs
StatePublished - Dec 2019

Keywords

  • Chlorella sp.
  • Liquid Triphasic Flotation
  • Microalgae
  • Protein extraction
  • Recycling

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