An amperometric biosensor based on direct immobilization of horseradish peroxidase on electrochemically reduced graphene oxide modified screen printed carbon electrode

Selvakumar Palanisamy, Binesh Unnikrishnan, Shen Ming Chen

    Research output: Contribution to journalArticlepeer-review

    23 Scopus citations

    Abstract

    In this work we report the synthesis of graphene oxide - horseradish peroxidase biocomposite (GO-HRP) by a solution method without using any cross-linkers. Then, the GO-HRP biocomposite was drop casted on a screen printed carbon electrode (SPCE) followed by the electrochemical reduction to reduced graphene oxide-HRP (ERGO-HRP) composite. The ERGO-HRP modified SPCE showed good electroanalytical properties towards the amperometric determination of H2O2. The film has been characterized by scanning electron microscopy (SEM) and electrochemical impedance spectroscopy (EIS). Both the studies revealed the effective immobilization of HRP on ERGO. The SPCE/ERGO-HRP showed a linear range of detection of 9 - 195 μM. The electrode also detects H2O2 in real sample solutions with appreciable accuracy.

    Original languageBritish English
    Pages (from-to)7935-7947
    Number of pages13
    JournalInternational Journal of Electrochemical Science
    Volume7
    Issue number9
    StatePublished - 2012

    Keywords

    • Amperometry
    • Graphene oxide
    • HO
    • Horseradish peroxidase
    • Immobilization
    • Reduced graphene oxide

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