15 Scopus citations


We have developed an efficient and inexpensive pipeline for streamlining large-scale collection and genome sequencing of bacterial isolates. Evaluation of this method involved a worldwide research collaboration focused on the model organism Salmonella enterica, the 10KSG consortium. Following the optimization of a logistics pipeline that involved shipping isolates as thermolysates in ambient conditions, the project assembled a diverse collection of 10,419 isolates from low- and middle-income countries. The genomes were sequenced using the LITE pipeline for library construction, with a total reagent cost of less than USD$10 per genome. Our method can be applied to other large bacterial collections to underpin global collaborations.

Original languageBritish English
Article number349
JournalGenome Biology
Issue number1
StatePublished - Dec 2021


  • iNTS
  • Salmonella
  • Thermolysates
  • Whole-genome sequencing


Dive into the research topics of 'An accessible, efficient and global approach for the large-scale sequencing of bacterial genomes'. Together they form a unique fingerprint.

Cite this