Alternating magnetic field plate for enhanced magnetofection of iron oxide nanoparticle conjugated nucleic acids

Murat Kaya Yapici, Ahmad Al Nabulsi, Nahla Rizk, Selwa Mokhtar Boularaoui, Nicolas Christoforou, Sungmun Lee

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

Magnet-assisted transfection or magnetofection refers to the delivery of nucleic acids to target cells with the help of conjugated superparamagnetic iron oxide nanoparticles (SPIONs) and external magnetic fields generated by permanent magnet plates. The external magnetic field, among other parameters, directly affects the transfection efficiency. However, standard permanent magnet plates generate static magnetic fields which are not as effective compared to time-varying, dynamic fields. In this work, we show a new and novel “AC magnet plate” compatible with standard 96-well cell culture plates, which can be easily adapted for benchtop use in a typical lab setting. We provide full design details, modelling, fabrication, measurement and testing on human embryonic kidney cells (HEK 293) to show transfection improvement. We perform magnetofection under different field conditions and show that with increasing AC content, efficiency of transfection is improved.

Original languageBritish English
Pages (from-to)598-605
Number of pages8
JournalJournal of Magnetism and Magnetic Materials
Volume469
DOIs
StatePublished - 1 Jan 2019

Keywords

  • AC
  • Alternating field
  • Dynamic field
  • Electromagnet
  • Gene delivery
  • HEK 293
  • Magnet plate
  • Magnetic transfection
  • Magnetofection
  • Nucleic acid
  • Oscillating field
  • SPION
  • Superparamagnetic iron oxide nanoparticle

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