Age-related alterations in cytochrome c-mediated caspase activation in rhesus macaque monkey (Macaca mulatta) brains

Aiqun Zhang, Dietrich E. Lorke, Helen W.L. Lai, Xiangyang Chu, Yan Wu, David T. Yew

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

Age-related changes and regional differences in caspase expression were determined in the primate brain. Using immunoblot analysis, the levels of endogenous caspase-3, caspase-9 and cytochrome c-triggered activated caspase-3 were examined in brain homogenates from the prefrontal, motor and visual cortices, cerebellum, hippocampus and amygdala of 4-year-old and 20-year-old rhesus macaques. Procaspase-3 was detected in similar quantities in all brain regions of both young and aging macaques. Being found in all brain regions, caspase-9 was significantly elevated in old macaques as compared to young ones. After incubation with cytochrome c, active forms of caspase-3 were detected in all brain regions of young and old macaques. In almost all brain regions of old monkeys, the levels of cytochrome c-dependent caspase-3 activation were higher than those of young macaques. These results suggest that the aging rhesus macaque brain has a lower threshold to apoptotic stimuli.

Original languageBritish English
Pages (from-to)112-120
Number of pages9
JournalMolecular Brain Research
Volume123
Issue number1-2
DOIs
StatePublished - 7 Apr 2004

Keywords

  • Aging
  • Aging process
  • Apoptosis
  • Caspase-3
  • Cerebral cortex
  • Cytochrome c
  • Development and regeneration
  • Primate

Fingerprint

Dive into the research topics of 'Age-related alterations in cytochrome c-mediated caspase activation in rhesus macaque monkey (Macaca mulatta) brains'. Together they form a unique fingerprint.

Cite this