Adjuvants in the liquid biphasic system

Sze Ying Lee, Pau Loke Show

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review


The liquid biphasic system (LBS) is a biocompatible purification technique, which consists of two immiscible aqueous-rich phases formed by a combination of polymer-polymer, polymer-salt, or salt-salt. Polymer-based LBSs have been extensively studied for the separation and purification of biomolecules. However, polymer-based LBSs exhibit a limited difference in polarities between the two aqueous phases, and thus their applications are restricted. In view of this constraint, ionic liquids are introduced as adjuvants in the polymer-based LBS, aiming to increase the polarity range between the two aqueous phases by modifying the hydrophobicity of the polymer-rich aqueous phase. The addition of ionic liquids as adjuvants in polymer-based LBSs alters the original ternary phase diagram and partitioning behavior of the biomolecules.

Original languageBritish English
Title of host publicationLiquid Biphasic System
Subtitle of host publicationFundamentals and Applications in Bioseparation Technology
Number of pages20
ISBN (Electronic)9780128199862
StatePublished - 1 Jan 2020


  • Adjuvant
  • Ionic liquid
  • Liquid biphasic system
  • Purification


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