Adjuvants in the liquid biphasic system

Sze Ying Lee; Pau Loke Show

doi:10.1016/B978-0-12-819986-2.00006-2

Adjuvants in the liquid biphasic system

Sze Ying Lee, Pau Loke Show

Chemical and Petroleum Engineering

Universiti Tunku Abdul Rahman

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

Abstract

The liquid biphasic system (LBS) is a biocompatible purification technique, which consists of two immiscible aqueous-rich phases formed by a combination of polymer-polymer, polymer-salt, or salt-salt. Polymer-based LBSs have been extensively studied for the separation and purification of biomolecules. However, polymer-based LBSs exhibit a limited difference in polarities between the two aqueous phases, and thus their applications are restricted. In view of this constraint, ionic liquids are introduced as adjuvants in the polymer-based LBS, aiming to increase the polarity range between the two aqueous phases by modifying the hydrophobicity of the polymer-rich aqueous phase. The addition of ionic liquids as adjuvants in polymer-based LBSs alters the original ternary phase diagram and partitioning behavior of the biomolecules.

Original language	British English
Title of host publication	Liquid Biphasic System
Subtitle of host publication	Fundamentals and Applications in Bioseparation Technology
Publisher	Elsevier
Pages	85-104
Number of pages	20
ISBN (Electronic)	9780128199862
DOIs	https://doi.org/10.1016/B978-0-12-819986-2.00006-2
State	Published - 1 Jan 2020

Keywords

Adjuvant
Ionic liquid
Liquid biphasic system
Purification

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10.1016/B978-0-12-819986-2.00006-2

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abstract = "The liquid biphasic system (LBS) is a biocompatible purification technique, which consists of two immiscible aqueous-rich phases formed by a combination of polymer-polymer, polymer-salt, or salt-salt. Polymer-based LBSs have been extensively studied for the separation and purification of biomolecules. However, polymer-based LBSs exhibit a limited difference in polarities between the two aqueous phases, and thus their applications are restricted. In view of this constraint, ionic liquids are introduced as adjuvants in the polymer-based LBS, aiming to increase the polarity range between the two aqueous phases by modifying the hydrophobicity of the polymer-rich aqueous phase. The addition of ionic liquids as adjuvants in polymer-based LBSs alters the original ternary phase diagram and partitioning behavior of the biomolecules.",

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AB - The liquid biphasic system (LBS) is a biocompatible purification technique, which consists of two immiscible aqueous-rich phases formed by a combination of polymer-polymer, polymer-salt, or salt-salt. Polymer-based LBSs have been extensively studied for the separation and purification of biomolecules. However, polymer-based LBSs exhibit a limited difference in polarities between the two aqueous phases, and thus their applications are restricted. In view of this constraint, ionic liquids are introduced as adjuvants in the polymer-based LBS, aiming to increase the polarity range between the two aqueous phases by modifying the hydrophobicity of the polymer-rich aqueous phase. The addition of ionic liquids as adjuvants in polymer-based LBSs alters the original ternary phase diagram and partitioning behavior of the biomolecules.

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KW - Ionic liquid

KW - Liquid biphasic system

KW - Purification

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BT - Liquid Biphasic System

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Adjuvants in the liquid biphasic system

Abstract

Keywords

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