A sustainable approach to derive sheep corneal scaffolds from stored slaughterhouse waste

Zehara M. Ali; Xinyu Wang; Meklit G. Shibru; Maha Alhosani; Nouf Alfadhli; Aysha Alnuaimi; Fiza F. Murtaza; Aisha Zaid; Rodaina Khaled; Ahmed E. Salih; Hema Vurivi; Sayel Daoud; Haider Butt; Vincent Chan; Igor V. Pantic; Jovana Paunovic; Peter R. Corridon

doi:10.1080/17460751.2024.2357499

A sustainable approach to derive sheep corneal scaffolds from stored slaughterhouse waste

Zehara M. Ali, Xinyu Wang, Meklit G. Shibru, Maha Alhosani, Nouf Alfadhli, Aysha Alnuaimi, Fiza F. Murtaza, Aisha Zaid, Rodaina Khaled, Ahmed E. Salih, Hema Vurivi, Sayel Daoud, Haider Butt, Vincent Chan, Igor V. Pantic, Jovana Paunovic, Peter R. Corridon

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Aim: The escalating demand for corneal transplants significantly surpasses the available supply. To bridge this gap, we concentrated on ethical and sustainable corneal grafting sources. Our objective was to create viable corneal scaffolds from preserved slaughterhouse waste. Materials & methods: Corneas were extracted and decellularized from eyeballs that had been refrigerated for several days. These scaffolds underwent evaluation through DNA quantification, histological analysis, surface tension measurement, light propagation testing, and tensile strength assessment. Results: Both the native and acellular corneas (with ~90% DNA removed using a cost-effective and environmentally friendly surfactant) maintained essential optical and biomechanical properties for potential clinical use. Conclusion: Our method of repurposing slaughterhouse waste, stored at 4°C for several days, to develop corneal scaffolds offers a sustainable and economical alternative xenograft model.

Original language	British English
Pages (from-to)	303-315
Number of pages	13
Journal	Regenerative Medicine
Volume	19
Issue number	6
DOIs	https://doi.org/10.1080/17460751.2024.2357499
State	Published - 2024

Keywords

Corneal transplantation
corneal xenograft
dECM
slaughterhouse waste
sustainable tissue engineering
xenotransplantation

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10.1080/17460751.2024.2357499

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Ali, Z. M., Wang, X., Shibru, M. G., Alhosani, M., Alfadhli, N., Alnuaimi, A., Murtaza, F. F., Zaid, A., Khaled, R., Salih, A. E., Vurivi, H., Daoud, S., Butt, H., Chan, V., Pantic, I. V., Paunovic, J., & Corridon, P. R. (2024). A sustainable approach to derive sheep corneal scaffolds from stored slaughterhouse waste. Regenerative Medicine, 19(6), 303-315. https://doi.org/10.1080/17460751.2024.2357499

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title = "A sustainable approach to derive sheep corneal scaffolds from stored slaughterhouse waste",

abstract = "Aim: The escalating demand for corneal transplants significantly surpasses the available supply. To bridge this gap, we concentrated on ethical and sustainable corneal grafting sources. Our objective was to create viable corneal scaffolds from preserved slaughterhouse waste. Materials & methods: Corneas were extracted and decellularized from eyeballs that had been refrigerated for several days. These scaffolds underwent evaluation through DNA quantification, histological analysis, surface tension measurement, light propagation testing, and tensile strength assessment. Results: Both the native and acellular corneas (with ~90% DNA removed using a cost-effective and environmentally friendly surfactant) maintained essential optical and biomechanical properties for potential clinical use. Conclusion: Our method of repurposing slaughterhouse waste, stored at 4°C for several days, to develop corneal scaffolds offers a sustainable and economical alternative xenograft model.",

keywords = "Corneal transplantation, corneal xenograft, dECM, slaughterhouse waste, sustainable tissue engineering, xenotransplantation",

author = "Ali, {Zehara M.} and Xinyu Wang and Shibru, {Meklit G.} and Maha Alhosani and Nouf Alfadhli and Aysha Alnuaimi and Murtaza, {Fiza F.} and Aisha Zaid and Rodaina Khaled and Salih, {Ahmed E.} and Hema Vurivi and Sayel Daoud and Haider Butt and Vincent Chan and Pantic, {Igor V.} and Jovana Paunovic and Corridon, {Peter R.}",

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year = "2024",

doi = "10.1080/17460751.2024.2357499",

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pages = "303--315",

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Ali, ZM, Wang, X, Shibru, MG, Alhosani, M, Alfadhli, N, Alnuaimi, A, Murtaza, FF, Zaid, A, Khaled, R, Salih, AE, Vurivi, H, Daoud, S, Butt, H , Chan, V, Pantic, IV, Paunovic, J & Corridon, PR 2024, 'A sustainable approach to derive sheep corneal scaffolds from stored slaughterhouse waste', Regenerative Medicine, vol. 19, no. 6, pp. 303-315. https://doi.org/10.1080/17460751.2024.2357499

TY - JOUR

T1 - A sustainable approach to derive sheep corneal scaffolds from stored slaughterhouse waste

AU - Ali, Zehara M.

AU - Wang, Xinyu

AU - Shibru, Meklit G.

AU - Alhosani, Maha

AU - Alfadhli, Nouf

AU - Alnuaimi, Aysha

AU - Murtaza, Fiza F.

AU - Zaid, Aisha

AU - Khaled, Rodaina

AU - Salih, Ahmed E.

AU - Vurivi, Hema

AU - Daoud, Sayel

AU - Butt, Haider

AU - Chan, Vincent

AU - Pantic, Igor V.

AU - Paunovic, Jovana

AU - Corridon, Peter R.

PY - 2024

Y1 - 2024

N2 - Aim: The escalating demand for corneal transplants significantly surpasses the available supply. To bridge this gap, we concentrated on ethical and sustainable corneal grafting sources. Our objective was to create viable corneal scaffolds from preserved slaughterhouse waste. Materials & methods: Corneas were extracted and decellularized from eyeballs that had been refrigerated for several days. These scaffolds underwent evaluation through DNA quantification, histological analysis, surface tension measurement, light propagation testing, and tensile strength assessment. Results: Both the native and acellular corneas (with ~90% DNA removed using a cost-effective and environmentally friendly surfactant) maintained essential optical and biomechanical properties for potential clinical use. Conclusion: Our method of repurposing slaughterhouse waste, stored at 4°C for several days, to develop corneal scaffolds offers a sustainable and economical alternative xenograft model.

AB - Aim: The escalating demand for corneal transplants significantly surpasses the available supply. To bridge this gap, we concentrated on ethical and sustainable corneal grafting sources. Our objective was to create viable corneal scaffolds from preserved slaughterhouse waste. Materials & methods: Corneas were extracted and decellularized from eyeballs that had been refrigerated for several days. These scaffolds underwent evaluation through DNA quantification, histological analysis, surface tension measurement, light propagation testing, and tensile strength assessment. Results: Both the native and acellular corneas (with ~90% DNA removed using a cost-effective and environmentally friendly surfactant) maintained essential optical and biomechanical properties for potential clinical use. Conclusion: Our method of repurposing slaughterhouse waste, stored at 4°C for several days, to develop corneal scaffolds offers a sustainable and economical alternative xenograft model.

KW - Corneal transplantation

KW - corneal xenograft

KW - dECM

KW - slaughterhouse waste

KW - sustainable tissue engineering

KW - xenotransplantation

UR - http://www.scopus.com/inward/record.url?scp=85195460678&partnerID=8YFLogxK

U2 - 10.1080/17460751.2024.2357499

DO - 10.1080/17460751.2024.2357499

M3 - Article

AN - SCOPUS:85195460678

SN - 1746-0751

VL - 19

SP - 303

EP - 315

JO - Regenerative Medicine

JF - Regenerative Medicine

IS - 6

ER -

A sustainable approach to derive sheep corneal scaffolds from stored slaughterhouse waste

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Keywords

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