A snake toxin against muscarinic acetylcholine receptors: Amino acid sequence, subtype specificity and effect on guinea-pig ileum

Mikael Jolkkonen, Abdu Adem, Ulf Hellman, Christer Wernstedt, Evert Karlsson

Research output: Contribution to journalArticlepeer-review

31 Scopus citations

Abstract

The sequence of muscarinic toxin 1 (MT1) from Dendroaspis angusticeps (green mamba) was determined (66 amino acids, Mr 7509). The central part, peptide 25-40, is rich in hydrophobic amino acids, which is a characteristic of muscarinic toxins. MT1 started to inhibit [3H]-NMS (N-methylscopolamine) binding to synaptosomal membranes of porcine brain (contains all five receptor subtypes) at about 1 nM and to membranes from pig heart muscle (only subtype m2) at about 1 μM. Binding of [3H]-AF-DX 384 to heart was inhibited with an ic50 of 14 μM and to brain in two steps. In the first step (IC50 = 32 nM) binding decreased by 37%, indicating that the toxin acted on m1 or m4 receptors, each accounting for about 40% of total receptor content. The second step was similar to the effect on heart. Pirenzepine inhibited binding of [125I]-MT1 to brain receptors with an ic50 of 6.5 nM, corresponding to a Ki, of about 6 nM. Literature values of Ki for pirenzepine are 16-18 nM for m1 and ≥ 120 mM for other subtypes. This indicates binding to m1 receptors. [125I]-MT1 bound to brain with a Kd of 20 nM and a Hill coefficient of 1.0 i.e. one toxin molecule per receptor. In guinea-pig ileum, MT1 (670 nM) produced a rapid contraction, reversible by atropine. The toxin may be an agonist, but might also cause contraction by inducing acetylcholine release by a different mechanism.

Original languageBritish English
Pages (from-to)399-410
Number of pages12
JournalToxicon
Volume33
Issue number4
DOIs
StatePublished - Apr 1995

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