A redox proteomic investigation of oxidative stress caused by benzoylecgonine in the freshwater bivalve Dreissena polymorpha

Alessandra Pedriali, Consuelo Riva, Marco Parolini, Simone Cristoni, David Sheehan, Andrea Binelli

Research output: Contribution to journalArticlepeer-review

31 Scopus citations

Abstract

Drugs of abuse and their human metabolites have been recently recognized as emerging environmental contaminants. Notwithstanding the fact that these kinds of compounds share some features with pharmaceuticals, their ecotoxicology has not yet been extensively investigated, although some of their characteristics may potentially threaten aquatic ecosystems. One of the most abundant drugs found in rivers and wastewaters is benzoylecgonine (BE), the main metabolite of cocaine. We applied a redox proteomics approach to evaluate changes in the proteome of Dreissena polymorpha exposed to two different concentrations of BE (0.5 and 1μg/l). Exposures were performed in vivo for a period of 14days and the effect of oxidative stress on protein thiol and carbonyl groups in mussel gills were evaluated. One-dimensional electrophoresis did not reveal a reduction in protein thiol content but showed a significant increase of protein carbonylation at both doses tested. Then, protein profiling using two-dimensional gel electrophoresis was performed with subsequent matrix assisted laser desorption/ionization time-of-flight (MALDI-TOF) and TOF/TOF with LIFT technique and linear ion trap combined with orbitrap mass spectrometer (LTQ-Orbitrap). This yielded de novo protein sequences suitable for database searching. These preliminary results and protein identifications obtained suggest that BE causes oxidative stress. Oxidative modifications were detected in differing classes of proteins such as those of the cytoskeleton, energetic metabolism and stress response.

Original languageBritish English
Pages (from-to)646-656
Number of pages11
JournalDrug Testing and Analysis
Volume5
Issue number8
DOIs
StatePublished - Aug 2013

Keywords

  • Benzoylecgonine
  • Dreissena polymorpha
  • Protein carbonyl
  • Protein thiol
  • Redox proteomics

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