A novel lipids recovery strategy for biofuels generation on microalgae Chlorella cultivation with waste molasses

Guo Yong Yew, Kuan Shiong Khoo, Wen Yi Chia, Yeek Chia Ho, Chung Lim Law, Hui Yi Leong, Pau Loke Show

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35 Scopus citations

Abstract

This study demonstrated a novel approach of lipids extraction with simultaneous biofuels conversion on microalgae Chlorella sorokiniana CY-1 cultivation using waste molasses as culture medium. The lipid extraction process was based on a hybrid liquid biphasic system (HLBS) which comprises of disruptive and extractive solvents in a single system. The microalgae cultivation with waste molasses showed a reduction in the chemical oxygen demand (COD), total suspended solid (TSS) and nitrate content on the waste medium (61.48 %, 63.94 % and 63.79 %, respectively). Additionally, a prompt lipids extraction with a recovery of 92.84 % and a cell disruption enhancement of 8.3-fold in the organic solvent phase was attained. The acid-treated C. sorokiniana CY-1 biomass showed a composition of carbon at 61.3 %, oxygen at 33.2 %, nitrogen at 5.5 % and potassium at 0.12 %. Sulphur content was not detected in the microalgae biomass and this had aided the production of fatty acids methyl ester (FAME) which was observed by a gas chromatographic-flame ionisation detector (GC-FID) profile. A disruptive-extraction method using acid assisted phase on HLBS showed potential for direct conversion of lipids into FAME to produce biofuels for engine combustion.

Original languageBritish English
Article number101665
JournalJournal of Water Process Engineering
Volume38
DOIs
StatePublished - Dec 2020

Keywords

  • Acid disruption
  • Biofuels
  • Cell wall
  • Lipids recovery
  • Microalgae

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