A microfluidic platform with castellated electrodes to separate cancer cells from blood cells

Abdulla Al-Ali; Waqas Waheed; Eiyad Abu-Nada; Bobby Mathew; Hyung Jin Sung; Anas Alazzam

doi:10.1109/MARSS49294.2020.9307849

A microfluidic platform with castellated electrodes to separate cancer cells from blood cells

Abdulla Al-Ali
, Waqas Waheed
, Eiyad Abu-Nada
, Bobby Mathew
, Hyung Jin Sung
, Anas Alazzam

Mechanical & Nuclear Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

5 Scopus citations

Abstract

This paper introduces a dielectrophoresis-based platform for the separation of cancer cells from blood cells. The platform consists of a two-layered device: a glass wafer with embedded castellated electrodes and a polydimethylsiloxane (PDMS) channel bonded on top of the glass wafer. The castellated electrodes generate a non-uniform electric field and exert dielectrophoretic force on cells suspended in a low-conductivity medium. The magnitude and direction of the force acting on different cells depend on the applied frequency along with difference in the dielectric properties of each type of cells and the suspension medium. In the experiment, a mixed sample of blood cells and MDA-MB-231 breast cancer cells, both suspended in a low conductive isotonic solution, were injected into the microchannel using a syringe pump. The applied frequency is tuned in such a way that the MDA-MB-231 cancer cells were attracted towards electrode tips whereas the blood cells were repelled away from the electrode tips to achieve successful separation.

Original language	British English
Title of host publication	Proceedings of MARSS 2020
Subtitle of host publication	International Conference on Manipulation, Automation, and Robotics at Small Scales
Editors	Sinan Haliyo, Mokrane Boudaoud, Albert Sill, Sergej Fatikow
Publisher	Institute of Electrical and Electronics Engineers Inc.
ISBN (Electronic)	9781728193496
DOIs	https://doi.org/10.1109/MARSS49294.2020.9307849
State	Published - 13 Jul 2020
Event	2020 International Conference on Manipulation, Automation, and Robotics at Small Scales, MARSS 2020 - Toronto, Canada Duration: 13 Jul 2020 → 17 Jul 2020

Publication series

Name	Proceedings of MARSS 2020: International Conference on Manipulation, Automation, and Robotics at Small Scales

Conference

Conference	2020 International Conference on Manipulation, Automation, and Robotics at Small Scales, MARSS 2020
Country/Territory	Canada
City	Toronto
Period	13/07/20 → 17/07/20

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

blood cells
cancer cells
castellated electrodes
dielectrophoresis
lab on a chip
microchannel
microfluidics
non-uniform electrical field

Access to Document

10.1109/MARSS49294.2020.9307849

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Al-Ali, A., Waheed, W., Abu-Nada, E., Mathew, B., Sung, H. J., & Alazzam, A. (2020). A microfluidic platform with castellated electrodes to separate cancer cells from blood cells. In S. Haliyo, M. Boudaoud, A. Sill, & S. Fatikow (Eds.), Proceedings of MARSS 2020: International Conference on Manipulation, Automation, and Robotics at Small Scales Article 9307849 (Proceedings of MARSS 2020: International Conference on Manipulation, Automation, and Robotics at Small Scales). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/MARSS49294.2020.9307849

Al-Ali, Abdulla ; Waheed, Waqas ; Abu-Nada, Eiyad et al. / A microfluidic platform with castellated electrodes to separate cancer cells from blood cells. Proceedings of MARSS 2020: International Conference on Manipulation, Automation, and Robotics at Small Scales. editor / Sinan Haliyo ; Mokrane Boudaoud ; Albert Sill ; Sergej Fatikow. Institute of Electrical and Electronics Engineers Inc., 2020. (Proceedings of MARSS 2020: International Conference on Manipulation, Automation, and Robotics at Small Scales).

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title = "A microfluidic platform with castellated electrodes to separate cancer cells from blood cells",

abstract = "This paper introduces a dielectrophoresis-based platform for the separation of cancer cells from blood cells. The platform consists of a two-layered device: a glass wafer with embedded castellated electrodes and a polydimethylsiloxane (PDMS) channel bonded on top of the glass wafer. The castellated electrodes generate a non-uniform electric field and exert dielectrophoretic force on cells suspended in a low-conductivity medium. The magnitude and direction of the force acting on different cells depend on the applied frequency along with difference in the dielectric properties of each type of cells and the suspension medium. In the experiment, a mixed sample of blood cells and MDA-MB-231 breast cancer cells, both suspended in a low conductive isotonic solution, were injected into the microchannel using a syringe pump. The applied frequency is tuned in such a way that the MDA-MB-231 cancer cells were attracted towards electrode tips whereas the blood cells were repelled away from the electrode tips to achieve successful separation.",

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author = "Abdulla Al-Ali and Waqas Waheed and Eiyad Abu-Nada and Bobby Mathew and Sung, \{Hyung Jin\} and Anas Alazzam",

note = "Funding Information: Acknowledgements This publication is based upon work supported by the Khalifa University of Science and Technology under Award No. [CIRA-2019-014]. Publisher Copyright: {\textcopyright} 2020 IEEE.; 2020 International Conference on Manipulation, Automation, and Robotics at Small Scales, MARSS 2020 ; Conference date: 13-07-2020 Through 17-07-2020",

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Al-Ali, A, Waheed, W, Abu-Nada, E, Mathew, B, Sung, HJ & Alazzam, A 2020, A microfluidic platform with castellated electrodes to separate cancer cells from blood cells. in S Haliyo, M Boudaoud, A Sill & S Fatikow (eds), Proceedings of MARSS 2020: International Conference on Manipulation, Automation, and Robotics at Small Scales., 9307849, Proceedings of MARSS 2020: International Conference on Manipulation, Automation, and Robotics at Small Scales, Institute of Electrical and Electronics Engineers Inc., 2020 International Conference on Manipulation, Automation, and Robotics at Small Scales, MARSS 2020, Toronto, Canada, 13/07/20. https://doi.org/10.1109/MARSS49294.2020.9307849

A microfluidic platform with castellated electrodes to separate cancer cells from blood cells. / Al-Ali, Abdulla; Waheed, Waqas; Abu-Nada, Eiyad et al.
Proceedings of MARSS 2020: International Conference on Manipulation, Automation, and Robotics at Small Scales. ed. / Sinan Haliyo; Mokrane Boudaoud; Albert Sill; Sergej Fatikow. Institute of Electrical and Electronics Engineers Inc., 2020. 9307849 (Proceedings of MARSS 2020: International Conference on Manipulation, Automation, and Robotics at Small Scales).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - A microfluidic platform with castellated electrodes to separate cancer cells from blood cells

AU - Al-Ali, Abdulla

AU - Waheed, Waqas

AU - Abu-Nada, Eiyad

AU - Mathew, Bobby

AU - Sung, Hyung Jin

AU - Alazzam, Anas

N1 - Funding Information: Acknowledgements This publication is based upon work supported by the Khalifa University of Science and Technology under Award No. [CIRA-2019-014]. Publisher Copyright: © 2020 IEEE.

PY - 2020/7/13

Y1 - 2020/7/13

N2 - This paper introduces a dielectrophoresis-based platform for the separation of cancer cells from blood cells. The platform consists of a two-layered device: a glass wafer with embedded castellated electrodes and a polydimethylsiloxane (PDMS) channel bonded on top of the glass wafer. The castellated electrodes generate a non-uniform electric field and exert dielectrophoretic force on cells suspended in a low-conductivity medium. The magnitude and direction of the force acting on different cells depend on the applied frequency along with difference in the dielectric properties of each type of cells and the suspension medium. In the experiment, a mixed sample of blood cells and MDA-MB-231 breast cancer cells, both suspended in a low conductive isotonic solution, were injected into the microchannel using a syringe pump. The applied frequency is tuned in such a way that the MDA-MB-231 cancer cells were attracted towards electrode tips whereas the blood cells were repelled away from the electrode tips to achieve successful separation.

AB - This paper introduces a dielectrophoresis-based platform for the separation of cancer cells from blood cells. The platform consists of a two-layered device: a glass wafer with embedded castellated electrodes and a polydimethylsiloxane (PDMS) channel bonded on top of the glass wafer. The castellated electrodes generate a non-uniform electric field and exert dielectrophoretic force on cells suspended in a low-conductivity medium. The magnitude and direction of the force acting on different cells depend on the applied frequency along with difference in the dielectric properties of each type of cells and the suspension medium. In the experiment, a mixed sample of blood cells and MDA-MB-231 breast cancer cells, both suspended in a low conductive isotonic solution, were injected into the microchannel using a syringe pump. The applied frequency is tuned in such a way that the MDA-MB-231 cancer cells were attracted towards electrode tips whereas the blood cells were repelled away from the electrode tips to achieve successful separation.

KW - blood cells

KW - cancer cells

KW - castellated electrodes

KW - dielectrophoresis

KW - lab on a chip

KW - microchannel

KW - microfluidics

KW - non-uniform electrical field

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M3 - Conference contribution

AN - SCOPUS:85099551141

T3 - Proceedings of MARSS 2020: International Conference on Manipulation, Automation, and Robotics at Small Scales

BT - Proceedings of MARSS 2020

A2 - Haliyo, Sinan

A2 - Boudaoud, Mokrane

A2 - Sill, Albert

A2 - Fatikow, Sergej

PB - Institute of Electrical and Electronics Engineers Inc.

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ER -

Al-Ali A, Waheed W, Abu-Nada E, Mathew B, Sung HJ, Alazzam A. A microfluidic platform with castellated electrodes to separate cancer cells from blood cells. In Haliyo S, Boudaoud M, Sill A, Fatikow S, editors, Proceedings of MARSS 2020: International Conference on Manipulation, Automation, and Robotics at Small Scales. Institute of Electrical and Electronics Engineers Inc. 2020. 9307849. (Proceedings of MARSS 2020: International Conference on Manipulation, Automation, and Robotics at Small Scales). doi: 10.1109/MARSS49294.2020.9307849

A microfluidic platform with castellated electrodes to separate cancer cells from blood cells

Abstract

Publication series

Conference

UN SDGs

Keywords

Access to Document

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