A highly sensitive molecular structural probe applied to in situ biosensing of metabolites using PEDOT:PSS

Ellasia Tan, Anna Maria Pappa, Charalampos Pitsalidis, James Nightingale, Sebastian Wood, Fernando A. Castro, Róisín M. Owens, Ji Seon Kim

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

A large amount of research within organic biosensors is dominated by organic electrochemical transistors (OECTs) that use conducting polymers such as poly(3,4-ethylene dioxythiophene) doped with poly(styrenesulfonate) (PEDOT:PSS). Despite the recent advances in OECT-based biosensors, the sensing is solely reliant on the amperometric detection of the bioanalytes. This is typically accompanied by large undesirable parasitic electrical signals from the electroactive components in the electrolyte. Herein, we present the use of in situ resonance Raman spectroscopy to probe subtle molecular structural changes of PEDOT:PSS associated with its doping level. We demonstrate how such doping level changes of PEDOT:PSS can be used, for the first time, on operational OECTs for sensitive and selective metabolite sensing while simultaneously performing amperometric detection of the analyte. We test the sensitivity by molecularly sensing a lowest glucose concentration of 0.02 mM in phosphate-buffered saline solution. By changing the electrolyte to cell culture media, the selectivity of in situ resonance Raman spectroscopy is emphasized as it remains unaffected by other electroactive components in the electrolyte. The application of this molecular structural probe highlights the importance of developing biosensing probes that benefit from high sensitivity of the material's structural and electrical properties while being complimentary with the electronic methods of detection.

Original languageBritish English
Pages (from-to)291-299
Number of pages9
JournalBiotechnology and Bioengineering
Volume117
Issue number1
DOIs
StatePublished - 1 Jan 2020

Keywords

  • doping level
  • in situ
  • metabolite sensing
  • OECT
  • PEDOT:PSS
  • resonance Raman

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