A highly sensitive and selective enzymatic biosensor based on direct electrochemistry of hemoglobin at zinc oxide nanoparticles modified activated screen printed carbon electrode

Selvakumar Palanisamy, Chelladurai Karuppiah, Shen Ming Chen, Prakash Periakaruppan

    Research output: Contribution to journalArticlepeer-review

    26 Scopus citations

    Abstract

    A sensitive enzymatic biosensor has been developed for the detection of hydrogen peroxide (H2O2), nitrite (NO2- ) and trichloroacetic acid (TCA) by using hemoglobin (Hb) immobilized on activated screen printed carbon electrode (ASPCE) and zinc oxide (ZnO) composite. A pair of well defined redox peaks is observed with a heterogeneous electron transfer rate constant (Ks) of 5.27 s-1 for Hb at ASPCE/ZnO. The biosensor exhibits the detection of H2O2, TCA and NO-2 in the concentration range of 0.5-129.5 μmolL-1, 2.5-72.5 mmolL-1 and 0.2-674 μmolL-1 with the detection limit of 0.083 μmolL-1, 0.12 mmolL-1 and 0.069 μmolL-1, respectively.

    Original languageBritish English
    Pages (from-to)1984-1993
    Number of pages10
    JournalElectroanalysis
    Volume26
    Issue number9
    DOIs
    StatePublished - Sep 2014

    Keywords

    • Activated screen printed carbon electrode
    • Biosensors
    • Direct electrochemistry
    • Hemoglobin
    • Zinc oxide

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