A facile two step heat treatment strategy for development of bioceramic scaffolds for hard tissue engineering applications

Ali Farzin; Shabir Hassan; Somayeh Ebrahimi-Barough; A. Ai; E. Hasanzadeh; Arah Goodarzi; J. Ai

doi:10.1016/j.msec.2019.110009

A facile two step heat treatment strategy for development of bioceramic scaffolds for hard tissue engineering applications

Ali Farzin, Shabir Hassan, Somayeh Ebrahimi-Barough, A. Ai, E. Hasanzadeh, Arah Goodarzi, J. Ai

Biological Sciences

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

In the present study, a two-step sintering (TSS) method has been used to improve the mechanical properties, biocompatibility, drug release, and osteogenesis abilities of hardystonite (HT) ceramic scaffolds for tissue engineering and drug delivery applications. The average particle size of HT scaffold is kept lower than 80 nm and is reached higher than 130 nm by using two-step and conventional sintering methods, respectively. The compressive strengths of the prepared nanocrystalline HT scaffolds were found to be significantly higher than those of the micro-structure HT and currently available hydroxyapatite scaffolds. A comparative analysis of cell viability and live/dead staining of human mesenchymal stem cells (hMSCs) in nano- and micro-structured HT scaffolds and their drug release potentiation was carried out. The results showed that the nano-structured HT scaffolds have higher cell viability, biocompatibility and longer-term doxorubicin (DOX) release potential than the micro-structured ones. The results of quantitative real-time polymerase chain reaction (qRT-PCR) and immunohistochemistry (IHC) analyses showed that the expression of adhesion and differentiation supporting genes were significantly higher in nano-structured HT scaffolds as compared to the micro-structured ones. The results of qRT-PCR also showed that the mRNA expression level of ERK1/2 and P38 MAPK from hMSCs were significantly higher in nano-structured HT scaffolds than the micro-structured ones. These results potentially open new aspects for using nano-structured scaffolds in bone tissue engineering applications.

Original language	British English
Article number	110009
Journal	Materials Science and Engineering C
Volume	105
DOIs	https://doi.org/10.1016/j.msec.2019.110009
State	Published - Dec 2019

Keywords

Biocompatibility
Mesenchymal stem cell differentiation
Nano-structured hardystonite scaffold
Two-step sintering

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10.1016/j.msec.2019.110009

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title = "A facile two step heat treatment strategy for development of bioceramic scaffolds for hard tissue engineering applications",

abstract = "In the present study, a two-step sintering (TSS) method has been used to improve the mechanical properties, biocompatibility, drug release, and osteogenesis abilities of hardystonite (HT) ceramic scaffolds for tissue engineering and drug delivery applications. The average particle size of HT scaffold is kept lower than 80 nm and is reached higher than 130 nm by using two-step and conventional sintering methods, respectively. The compressive strengths of the prepared nanocrystalline HT scaffolds were found to be significantly higher than those of the micro-structure HT and currently available hydroxyapatite scaffolds. A comparative analysis of cell viability and live/dead staining of human mesenchymal stem cells (hMSCs) in nano- and micro-structured HT scaffolds and their drug release potentiation was carried out. The results showed that the nano-structured HT scaffolds have higher cell viability, biocompatibility and longer-term doxorubicin (DOX) release potential than the micro-structured ones. The results of quantitative real-time polymerase chain reaction (qRT-PCR) and immunohistochemistry (IHC) analyses showed that the expression of adhesion and differentiation supporting genes were significantly higher in nano-structured HT scaffolds as compared to the micro-structured ones. The results of qRT-PCR also showed that the mRNA expression level of ERK1/2 and P38 MAPK from hMSCs were significantly higher in nano-structured HT scaffolds than the micro-structured ones. These results potentially open new aspects for using nano-structured scaffolds in bone tissue engineering applications.",

keywords = "Biocompatibility, Mesenchymal stem cell differentiation, Nano-structured hardystonite scaffold, Two-step sintering",

author = "Ali Farzin and Shabir Hassan and Somayeh Ebrahimi-Barough and A. Ai and E. Hasanzadeh and Arah Goodarzi and J. Ai",

note = "Funding Information: The authors are grateful for the support to this research by the Harvard Medical School (HMS), Tehran University of Medical Sciences (TUMS), and Swiss National Science Foundation. This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors. Publisher Copyright: {\textcopyright} 2019 Elsevier B.V.",

year = "2019",

month = dec,

doi = "10.1016/j.msec.2019.110009",

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T1 - A facile two step heat treatment strategy for development of bioceramic scaffolds for hard tissue engineering applications

AU - Farzin, Ali

AU - Hassan, Shabir

AU - Ebrahimi-Barough, Somayeh

AU - Ai, A.

AU - Hasanzadeh, E.

AU - Goodarzi, Arah

AU - Ai, J.

N1 - Funding Information: The authors are grateful for the support to this research by the Harvard Medical School (HMS), Tehran University of Medical Sciences (TUMS), and Swiss National Science Foundation. This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors. Publisher Copyright: © 2019 Elsevier B.V.

PY - 2019/12

Y1 - 2019/12

N2 - In the present study, a two-step sintering (TSS) method has been used to improve the mechanical properties, biocompatibility, drug release, and osteogenesis abilities of hardystonite (HT) ceramic scaffolds for tissue engineering and drug delivery applications. The average particle size of HT scaffold is kept lower than 80 nm and is reached higher than 130 nm by using two-step and conventional sintering methods, respectively. The compressive strengths of the prepared nanocrystalline HT scaffolds were found to be significantly higher than those of the micro-structure HT and currently available hydroxyapatite scaffolds. A comparative analysis of cell viability and live/dead staining of human mesenchymal stem cells (hMSCs) in nano- and micro-structured HT scaffolds and their drug release potentiation was carried out. The results showed that the nano-structured HT scaffolds have higher cell viability, biocompatibility and longer-term doxorubicin (DOX) release potential than the micro-structured ones. The results of quantitative real-time polymerase chain reaction (qRT-PCR) and immunohistochemistry (IHC) analyses showed that the expression of adhesion and differentiation supporting genes were significantly higher in nano-structured HT scaffolds as compared to the micro-structured ones. The results of qRT-PCR also showed that the mRNA expression level of ERK1/2 and P38 MAPK from hMSCs were significantly higher in nano-structured HT scaffolds than the micro-structured ones. These results potentially open new aspects for using nano-structured scaffolds in bone tissue engineering applications.

AB - In the present study, a two-step sintering (TSS) method has been used to improve the mechanical properties, biocompatibility, drug release, and osteogenesis abilities of hardystonite (HT) ceramic scaffolds for tissue engineering and drug delivery applications. The average particle size of HT scaffold is kept lower than 80 nm and is reached higher than 130 nm by using two-step and conventional sintering methods, respectively. The compressive strengths of the prepared nanocrystalline HT scaffolds were found to be significantly higher than those of the micro-structure HT and currently available hydroxyapatite scaffolds. A comparative analysis of cell viability and live/dead staining of human mesenchymal stem cells (hMSCs) in nano- and micro-structured HT scaffolds and their drug release potentiation was carried out. The results showed that the nano-structured HT scaffolds have higher cell viability, biocompatibility and longer-term doxorubicin (DOX) release potential than the micro-structured ones. The results of quantitative real-time polymerase chain reaction (qRT-PCR) and immunohistochemistry (IHC) analyses showed that the expression of adhesion and differentiation supporting genes were significantly higher in nano-structured HT scaffolds as compared to the micro-structured ones. The results of qRT-PCR also showed that the mRNA expression level of ERK1/2 and P38 MAPK from hMSCs were significantly higher in nano-structured HT scaffolds than the micro-structured ones. These results potentially open new aspects for using nano-structured scaffolds in bone tissue engineering applications.

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KW - Mesenchymal stem cell differentiation

KW - Nano-structured hardystonite scaffold

KW - Two-step sintering

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VL - 105

JO - Materials Science and Engineering C

JF - Materials Science and Engineering C

M1 - 110009

ER -

A facile two step heat treatment strategy for development of bioceramic scaffolds for hard tissue engineering applications

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Keywords

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