Gel Permeation Chromatograph

Gel Permeation Chromatography (GPC), also known as Size Exclusion Chromatography (SEC), is a chromatographic method used to analyze and characterize the size and molecular weight distribution of polymers, proteins, and other macromolecules. Principle: In GPC, molecules are separated based on their hydrodynamic volume (or size in solution). Larger molecules elute from the column earlier than smaller molecules because they are excluded from entering the pores of the packing material, thus traveling a shorter path. Smaller molecules, on the other hand, enter the pores and take longer to elute. Components: Injection System; Columns; Detector; Mobile Phase & Pump Application: -Polymers: Determination of molecular weight distribution, average molecular weights, and polymer size. -Proteins and Biopolymers: Understanding size, shape, aggregation, and molecular weight. -Other Macromolecules: Like oligonucleotides or polysaccharides. It provides a rapid, reliable, and non-destructive means of analysis, making it a mainstay in both research and industry settings.